Project Description

This PhD project will develop and apply cutting-edge single-cell proteomics workflows to study circulating tumour cells (CTCs) from patients with small-cell lung cancer (SCLC). The successful candidate will work at the interface of analytical chemistry, molecular biology, and cancer research, using state-of-the-art mass spectrometry and liquid handling automation to profile the protein composition of individual tumour cells isolated from blood samples.

The project will build upon novel molecularly imprinted polymer (MIP)-based probes developed by MIPrecise partners to selectively capture and fluorescently label CTCs. Using advanced cell sorting and nano-volume sample preparation technologies, the PhD student will analyze the global proteomes of single CTCs to identify protein signatures linked to tumour aggressiveness and metastasis. These results will be integrated with matched single-cell transcriptomics data to define multiomic biomarkers that could enable earlier detection, better patient stratification, and more precise treatment of SCLC.

The PhD candidate will join the Schoof Lab at DTU Bioengineering, an international leader in single-cell proteomics, and collaborate closely with BAM, LNU, and UMU. The position offers extensive training in mass spectrometry, microfluidics, data analysis, and cancer biology within a vibrant, interdisciplinary European network focused on molecularly precise diagnostics.

Candidate requirements

You must have:

• A Master’s degree (or equivalent) in biotechnology, biochemistry, analytical chemistry, bioengineering, molecular biology, or a related field (120 ECTS points).
• Strong experimental skills in biochemistry, cell biology, or analytical workflows
• Interest in single-cell analysis, cancer biology, and translational research.
• Basic level expertise in computational biology (e.g., bioinformatics, machine learning), with a strong willingness to advance these skill sets on biological data.
• Ability to work independently and collaboratively within an international, interdisciplinary team.
• Good communication skills in English, both written and spoken.
• A curiosity-driven mindset and enthusiasm for developing and applying cutting-edge technologies to address biomedical questions.
• A proactive attitude towards learning and applying new skills.

It would further be beneficial if you have some of the following skills:

• Prior experience with mass spectrometry, proteomics, or microfluidics
• Experience with programming (Python preferred) for bioinformatics or data science applications.
• Demonstrated track record with single-cell technologies (experimental and/or data analytics)

Other information

DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility. Our group is at the forefront of single-cell and spatial proteomics, developing and applying advanced mass spectrometry and liquid handling technologies to study cellular heterogeneity and signaling in health and disease. We work closely with clinical and industrial partners to translate proteomic discoveries into diagnostic and therapeutic innovations, with current projects spanning hematology, oncology, and precision medicine.

Supervisor information

Associate Professor Erwin M. Schoof
DTU Bioengineering

https://www.bioengineering.dtu.dk/