Nationality: Greek
Background: I completed my B.Sc. in Chemistry and my M.Sc. in Bioanalysis – Pharmaceutical Analysis at Aristotle University of Thessaloniki, where I developed a strong interest in analytical chemistry and mass spectrometry. Throughout my studies, I had the opportunity to spend two semesters abroad at Julius Maximilians University of Würzburg and Humboldt University of Berlin. These experiences were invaluable in strengthening my practical laboratory skills, expanding my clinical research experience and enabling me to work in international and interdisciplinary environments.
My research interests: Bioanalytical chemistry, Mass spectrometry, Proteomics, Translational research
My PhD goals: My PhD goal is to develop and validate robust targeted mass spectrometry methods for the detection of cancer-related phosphopeptide biomarkers using molecularly imprinted polymers (MIPs) as selective enrichment tools. The project aims to improve phosphoproteomic analysis for precision oncology by creating sensitive, reproducible, and clinically applicable workflows for monitoring oncogenic signalling pathways in cancers such as small-cell lung cancer. At the same time, I aim to collaborate and exchange knowledge with amazing researchers from different disciplines within the MIPrecise network.
My hobbies: Classical guitar, Travelling, Cooking, Cinema, Learning German
My project in MIPrecise: Development and validation of multiplexed assays for biomarkers of oncogenic pathways
Master thesis: Development and validation of UPLC-MS/MS method for the determination of tacrolimus and bioactive plant-derived compounds in biological samples.
Tacrolimus is an immunosuppressive drug with a narrow therapeutic window, making regular monitoring of its blood concentrations essential to avoid adverse effects. It is also known that the consumption of grapefruit juice can affect the pharmacokinetics of tacrolimus by inhibiting the CYP3A4 enzyme, thereby increasing its bioavailability. In this study, a sensitive and selective UPLC-MS/MS method was developed and validated for the simultaneous quantification of tacrolimus and five grapefruit juice metabolites in whole blood, serum, and urine samples. The analytical workflow, including LC-MS/MS parameters and sample preparation, was extensively optimized to achieve high sensitivity, selectivity, and reproducibility. The method demonstrated excellent linearity, accuracy, precision according to international validation guidelines. Limits of detection (LOD) ranged from 0.002 to 0.4 ng/mL, while limits of quantification (LOQ) ranged from 0.008 to 1.33 ng/mL, confirming the suitability of the method for application in real patient samples. After successful validation, the method was applied to real biological samples from a patient who daily consumed 250 mL of grapefruit juice alongside tacrolimus. The results revealed an increase in tacrolimus blood levels during co-administration with the juice, indicating inhibition of the drug’s metabolism. Overall, this work highlighted the utility of developing specialized analytical methods for the pharmacokinetic monitoring of tacrolimus and for studying the impact of exogenous factors such as grapefruit juice, emphasizing the importance of a personalized therapeutic approach.